Cloning and expression of the cloacin DF13/aerobactin receptor of Escherichia coli (ColV-K30)
نویسندگان
چکیده
منابع مشابه
molecular cloning and expression of rat µ-opioid receptor in escherichia coli (bl21)
the µ (mu) opioid receptors, which mediate the effects of morphine, are widely distributed in brain. the purpose of this study was to design a simple expression system for rat µ-receptor in escherichia coli (bl21). in this laboratory study, rat µ-receptor cdna was isolated from pcdna3 vector using xba1 and hind3 restriction enzymes. pet-15b was digested by nco1 restriction enzyme. µ-receptor cd...
متن کاملCloning and Expression of Mannheimia haemolytica PlpE Gene in Escherichia coli and its Immunogenicity Assessment
Mannheimia haemolytica is responsible for considerable economic losses to cattle, sheep, and goat industries in many parts of the world. This bacterium isone of the causative agents of shipping fever in cattle. Current vaccines against M. haemolytica are moderately efficacious since they do not provide complete protection against the disease. Production of a...
متن کاملExpression Cloning of Recombinant Escherichia coli lacZ Genes Encoding Cytoplasmic and Nuclear P-galactosidase Variants
Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...
متن کاملCloning and expression of Eimeria necatrix microneme5 gene in Escherichia coli
Background: Coccidiosis caused by Eimeria necatrix has the most economic impact onpoultry production. Micronemal proteins in Eimeria necatrix are thoughtto be critical ligands determining host cell specificity at the time ofinvasion. OBJECTIVES: Isolation and purification of Eimeria necatrix oocysts from Khuzestan province of Iran was performed. AcDNA encoding microneme 5 (EnMIC5...
متن کاملCloning and Expression of Cyclophilin from Platanus orientalis Pollens in Escherichia coli
Background: Allergy is a clinical disorder affecting the human population with wide geographical distribution. Platanus orientalis (P. orientalis) trees are planted in many countries and their pollen causes allergic reactions. Cyclophilin has recently been identified as one of the most important allergens of P. orientalis pollen. We aimed to clone and purify this allergen in Escherichia coli...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1983
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.153.2.716-721.1983